Simultaneous Determination Of BPA And BPS Using UV/Vis Spectrophotometry And HPLC - The African Boss

Simultaneous Determination of BPA and BPS Using UV/Vis Spectrophotometry and HPLC

Message from the author of the thesis

Jean Eudes Benecyo

My name is Jean Eudes Benecyo and I am the author of this thesis which I wrote in 2016 as an honors graduation project. It has also been uploaded to my university website where it has gained a lot of attention with over 1000 downloads in only the first year since its upload to their website.

So I decided to add it to my website so we could have a better one on one communication so you can share what you like or any concerns you got from reading it.

I started this as a senior honors graduation project. But what started as a project ended up becoming something I fell in love with because of the impact the research had so I decided to put in the hard work to bring it to you in my thesis and hopefully you will enjoy the amount of information I researched and put together and the lab experiments I did.

Thank you and do not hesitate to contact me for more information. And visit the rest of my website and hopefully I can help you in more ways since I have put together my science, computer and business background to build this website.


Simultaneous Determination of BPA and BPS Using UV/Vis Spectrophotometry and HPLC


Jean Eudes Benecyo , Ouachita Baptist University

Date of Award


Document Type




First Advisor

Dr. Sara E. Hubbard

Second Advisor

Dr. Marty Perry

Third Advisor

Dr. Byron Eubanks


Bisphenol A (BPA) has been one of the most used plasticizers with more than 4.8 million tons produced in 2012. BPA is also an endocrine disruptor that has been linked to adverse health effects such as cancer, obesity, behavioral and mood changes, lowered fertility, developmental changes and more in humans and other animals. The evidence of the toxicity of BPA, even at very low levels, has caused many countries to limit its use, especially in baby bottles and other baby-related hard plastic items. In these times, BPA has been replaced with other bisphenols, such as Bisphenol S (BPS). BPS is more stable and heat resistant that BPA. However, BPS is also an endocrine disruptor and can behave like BPA in cellular activities. Also, studies have shown more dermal penetration of BPS than BPA, and it has been linked to similar adverse health effects.

In this research, methods were developed to simultaneously determine concentrations of BPA and BPS using UV/VIS Absorption Spectrophotometry and High Pressure liquid Chromatography (HPLC).

These methods were applied to water: methanol (1:1) samples exposed to different kinds of plastics, food cans and thermal receipt paper to test for leaching of BPA and/or BPS.

The concentrations determined ranged from w-x for BPA and y-z from BPS. The latter concentrations were above the Total Daily Intake approved by the Food and Drug Administration or the European Food Safety Authority. The methods used suggested that other chemicals leached out in addition to BPA and BPS. Time (0-2 weeks) and temperature (22-70 degrees C) were varied to simulated everyday use of these products.

The effectiveness of the UV-VIS Absorption Spectrophotometry in determining concentrations of BPA and BPS in samples was compared to HPLC using statistical analysis. And this method proved to be effective.

Please wait 0-4 seconds for the book to load below

Access this publication HERE

Download this publication HERE

Recommended Citation

Benecyo, Jean Eudes, “Simultaneous Determination of BPA and BPS Using UV/Vis Spectrophotometry and HPLC” (2016). Honors Theses. 233. 


Need some help?

Were you able to find Everything you are looking for?

Please schedule a time with our AI assistant, and I will be more than happy to answer all of your unanswered questions.